MicroRNA-208a Dysregulates Apoptosis Genes Expression and Promotes Cardiomyocyte Apoptosis during Ischemia and Its Silencing Improves Cardiac Function after Myocardial Infarction

Figure 2

miR-208a upregulation promotes cardiomyocyte apoptosis during ischemia and its silencing attenuates myocyte apoptosis (). (a) Representative TUNEL stained images of neonatal cardiomyocytes showing increased apoptosis following agomir treatment and decreased apoptosis following antagomir treatment and simulated myocyte ischemia (×20 objective). (b) Graph of percentage apoptosis following simulated ischemia of neonatal cardiomyocytes shows that miR-208a agomir significantly upregulated myocyte apoptosis while miR-208a antagomir transfection attenuated ischemia induced apoptosis ( per group). (c) Bax silencing using siRNA attenuated miR-208a agomir induced cardiomyocyte apoptosis during ischemia ( per group).

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