A Lipid Mediator Hepoxilin A3 Is a Natural Inducer of Neutrophil Extracellular Traps in Human Neutrophils

Figure 3

PBT-3 induces DNA release from human neutrophils. (a) Structure of PBT-3. (b) Neutrophils were seeded into 96-well plates in the presence or absence of PBT-3 (20 μg/mL), and the extracellular DNA release was monitored using Sytox Green cell impermeable DNA dye. The results show a time-dependent increase in NET release after activation with PBT-3 (20 μg/mL) (). R = Methyl.

(a)

(b)