Research Article

A Lipid Mediator Hepoxilin A3 Is a Natural Inducer of Neutrophil Extracellular Traps in Human Neutrophils

Figure 4

PBT-3 induces NETosis. Four hours after stimulation with either vehicle or PBT-3 (20 μg/mL), cells were fixed and stained for immunofluorescence analysis. Cells were stained for DNA (green) and MPO (red) after activation with PBT-3 (20 μg/mL). Images are representative of 3 independent experiments.