A Lipid Mediator Hepoxilin A3 Is a Natural Inducer of Neutrophil Extracellular Traps in Human Neutrophils

Figure 5

Hepoxilin A3-mediated NETosis is both NOX-dependent and NOX-independent depending on the dose of HxA3. A fluorescence plate reader assay was utilized to monitor NET DNA release upon stimulation with HxA3 in the presence or absence of the NOX inhibitor DPI (20 μM) (; ; compared to cells activated with HxA3 (2.5 μg/mL) in the presence of DPI ; ; (one-way ANOVA)).