Postmitotic Expression of SOD1^G93A Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation

Figure 2

Postmitotic expression of mutant SOD1 gene inhibits C2C12 cells differentiation. (a) Representative immunofluorescence images of C2C12 and C2C12 MLC/ cells stained with anti-myosin heavy chain (MHC) antibody after 5 days in differentiation medium. (b) The histograms represent the differentiation index (left panel) and fusion index (right panel) in control (white bars) and transfected cells (black bars). (c) Upper panel shows representative western blot analysis of MHC expression in C2C12 and C2C12 MLC/ cells. Lower panel shows densitometric analysis for MHC expression in C2C12 (white bars) and C2C12 MLC/ (black bars). D0, D2, and D5 referred to days of culture in differentiation condition. Data are represented as mean ± SEM ().

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