Postmitotic Expression of SOD1^G93A Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation

Figure 4

Muscle specific expression of human mutant SOD1 gene alters signaling pathways in muscle differentiation. (a) Representative western blot analysis for phospho-Akt (Thr 308) and total Akt expression (upper panel) and densitometric analysis (lower panel) of the ratio between phosphorylated Akt and the total form (lower panel) in C2C12 (white bars) and C2C12 MLC/ (black bars). (b) Representative western blot analysis for phospho-P70 (Thr 389) and total P70 expression (upper panel) and densitometric analysis (lower panel) of the ratio between phospho-P70 and total P70 (lower panel) in C2C12 (white bars) and C2C12 MLC/ (black bars). (c) Representative western blot analysis of phospho-p42/44 MAPK and p42/44 MAPK expression (upper panel) and densitometric analysis (lower panel) of the ratio between phospho-p42-44 MAPK and total p42/44 MAPK in C2C12 (white bars) and C2C12 MLC/ (black bars). D0, D2, and D5 referred to days of culture in differentiation condition. Data are represented as mean ± SEM ().

(a)

(b)

(c)