Notch Signaling Pathway Was Involved in Regulating Programmed Cell Death 1 Expression during Sepsis-Induced Immunosuppression

Figure 4

Notch inhibition by DAPT decreased PD-1 and IL-10 transcriptions in LPS-tolerant THP1 cells. LPS-tolerant THP1 cells were induced as mentioned above. In tolerant group, DAPT (40 μM) was added into the culture at the beginning of the treatment of LPS to inhibit the Notch signaling pathway. The mRNA levels of PD-1 (a), IL-10 (c), and TNF-α (d) and protein levels of PD-1 (b) were examined in control, LPS-nontolerant, LPS-tolerant, and DAPT groups. Data are normalized to β-actin mRNA and presented as . value < 0.05.