Chemerin15-Ameliorated Cardiac Ischemia-Reperfusion Injury Is Associated with the Induction of Alternatively Activated Macrophages

Figure 3

C15 generated alternatively activated macrophages in the ischemic myocardium. (a) Representative photographs of macrophage infiltration in the ischemic myocardium after 48 h of reperfusion. (b) The number of F4/80⁺ macrophages infiltrated into the ischemic myocardium was analyzed by flow cytometry (). (c) The number of classically activated M1 (iNOS⁺F4/80⁺) macrophages infiltrated into the ischemic myocardium was analyzed with flow cytometry (). (d) The number of alternatively activated M2 (CD206⁺F4/80⁺) macrophages that were infiltrated into the ischemic myocardium was analyzed with flow cytometry (). (e) M1 and M2 markers were analyzed with real-time PCR (). (f) The serum levels of proinflammatory cytokines (tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)) and anti-inflammatory cytokines (IL-10) were measured after 48 h of reperfusion (). < 0.05 versus the I/R or sham groups; < 0.05 versus the I/R group.

(a)

(b)

(c)

(d)

(e)

(f)