The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains

Figure 2

Ratio of internalized/adhered P. brasiliensis yeast cell by GM-BMMs and M-BMMs of A/J and B10.A mouse strains. Phagocytosis assays were performed employing a MOI (multiplicity of infection) of 5 : 1 macrophage to P. brasiliensis (Pb18) yeast cells, for incubation times of 6 h and 24 h. After the coculture, the cells were stained with Panotic kit. An average of 300 macrophages was counted and the number of ingested and/or adherent yeasts was determined. (, , , and ).