The Effects of Paracoccidioides brasiliensis Infection on GM-CSF- and M-CSF-Induced Mouse Bone Marrow-Derived Macrophage from Resistant and Susceptible Mice Strains

Table 1

Comparison of differentially expressed genes in GM-BMM versus M-BMM of Paracoccidioides brasiliensis infected A/J and B10.A mice.

Gene	Name	Fold changes (FC)
		A/J		B10.A
		M-BMM + Pb18 versus M-BMM	GM-BMM + Pb18 versus GM-BMM	M-BMM + Pb18 versus M-BMM	GM-BMM + Pb18 versus GM-BMM
Pattern of recognition receptor (PRR)
TLR2	Toll-like receptor 2	−1.7	1.4	3.7	−4.3
TLR4	Toll-like receptor 4	1.2	1.0	1.4	−6.3
TLR9	Toll-like receptor 9	−1.7	1.3	1.1	−9.1
Clec4n (Dectin-2)	C-type lectin domain family 4, member n	6.4	2.1	6.1	1.6
Clec7a (Dectin-1)	C-type lectin domain family 7, member a	5.3	2.1	4.4	1.1
Mrc1 (MR)	Mannose receptor, C-type 1	3.0	2.0	−1.7	−2.7
Nlrp3	NLR family, pyrin domain containing 3	1.3	2.9	4.8	−1.9
Scarf1	Scavenger receptor class F, member 1	3.8	−1.2	−1.7	−2.1
Itgb2	Integrin beta 2	−1.4	1.2	2.7	−3.0
Mbl2	Mannose-binding protein (protein C) 2	1.8	1.2	−20.2	5.5
CD14	CD14 antigen	2.0	3.0	−1.3	8.8
Itgam	Integrin alpha M	−1.4	1.6	1.3	−3.6
Colec12	Collectin subfamily member 12	4.6	1.8	3.0	−1.1
PRR signal Transduction
Casp-8	Caspase-8	1.5	3.9	1.3	1.6
Irak4	Interleukin-1 receptor-associated kinase 4	1.3	2.1	1.5	−2.6
Mapk14	Mitogen-activated protein kinase 14	1.1	1.3	2.3	−3.6
Mapk8	Mitogen-activated protein kinase 8	−1.7	1.4	2.0	−1.6
MyD88	Myeloid differentiation primary response gene 88	1.5	4.6	2.6	−3.0
Bcl10	B-cell leukemia/lymphoma 10	3.3	4.9	1.6	2.0
Malt1	Mucosa associated lymphoid tissue lymphoma translocation gene 1	2.4	1.7	3.1	−1.7
Pycard	PYD and CARD domain containing	−3.1	1.3	1.7	−5.5
Plcg2	Phospholipase C gamma 2	−2.2	−1.1	2.0	−4.9
Tirap (Mal)	Toll-interleukin-1 receptor (TIR) domain-containing adaptor protein	−1.1	1.3	1.4	−7.1
Raf1	V-raf-leukemia viral oncogene 1	1.1	1.1	3.2	−5.4
Syk	Spleen tyrosine kinase	1.5	1.3	2.9	−6.1
CD40	CD40 antigen	−1.4	10.2	2.0	10.9
Transcription factor and other proteins
Map2k4 (MKK4)	Mitogen-activated protein kinase 4	−1.3	1.8	1.9	−4.5
Mapk8	Mitogen-activated protein kinase 8	−1.7	1.4	2.0	−1.6
Nfkbia	Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha	−1.7	1.7	2.0	1.1
Cytokines
Csf2 (GM-CSF)	Colony stimulating factor 2 (granulocyte-macrophage)	−3.3	3.1	−2.0	−8.2
Il1a	Interleukin-1 alpha	5.4	15.0	4.5	5.8
Il1b	Interleukin-1 beta	1.8	31.4	3.4	7.0
Il2	Interleukin-2	1.3	124.7	−6.5	5.0
Il6	Interleukin-6	1.0	61.7	−1.4	4.4
Il10	Interleukin-10	−1.8	101.1	1.5	26.9
Il12a	Interleukin-12A	1.3	4.4	1.8	3.5
Il12b	Interleukin-12B	1.5	21.7	1.5	9.0
Il18	Interleukin-18	−1.2	2.4	1.0	−1.3
Il23a	Interleukin-23, alpha subunit p19	1.2	40.4	2.0	1.9
Tnf	Tumor necrosis factor alpha	−1.0	18.7	−1.1	18.6
Chemokines
Ccl5	Chemokine (C-C motif) ligand 5	1.1	6.2	3.5	21.1
Ccl12	Chemokine (C-C motif) ligand 12	−4.5	6.9	1.1	7.7
Ccl20	Chemokine (C-C motif) ligand 20	−1.1	6.0	2.0	1.0
Cxcl1 (KC)	Chemokine (C-X-C motif) ligand 1	8.8	53.9	6.5	19.4
Cxcl3	Chemokine (C-X-C motif) ligand 3	3.5	3.4	15.2	64.4
Cxcl9	Chemokine (C-X-C motif) ligand 9	2.1	3.9	1.5	3.7
Cxcl10 (IP-10)	Chemokine (C-X-C motif) ligand 10	−1.9	6.8	2.5	8.9
Cxcl11	Chemokine (C-X-C motif) ligand 11	2.1	−1.2	−2.7	−1.3
Other proteins
Ptgs2 (Cox-2)	Prostaglandin-endoperoxide synthase 2	8.3	28.0	18.8	11.3
Chia1	Chitinase, acidic	−1.7	−2.0	−3.4	−4.9
Stat1	Signal transducer and activator of transcription 1	−2.5	2.2	−1.1	−2.6
C3	Complement component 3	2.6	6.0	2.2	1.1
C5ar1	Complement component 5a receptor 1	3.4	2.7	2.8	1.5
Fcgr3	Fc receptor, IgG, low affinity III	4.9	1.4	1.5	−1.3

Genes In bold lettering had their transcript levels significantly modulated (FC ≥ 2 or ≤ −2 and value < 0.05 as described in Section 2). Positive and negative values represent genes with expression induced and repressed, respectively.