Carbon Monoxide Inhibits Tenascin-C Mediated Inflammation via IL-10 Expression in a Septic Mouse Model

Figure 3

CO inhibits LPS-induced TN-C expression and proinflammatory cytokines in peritoneal macrophages. (a-b) Macrophages were pretreated with CORM-2 (0, 10, 20, and 40 μM) for 1 h and then stimulated with LPS (100 ng/mL) for 8 h. TN-C, TNF-α, and IL-6 mRNA levels were detected by Real Time RT-PCR, respectively. (c-d) Cells were pretreated with CORM-2 (20 μM) or RuCl₃ (20 μM) for 1 h and stimulated with or without LPS (100 ng/mL) for 8 h. RuCl₃ was used as a negative control for CORM-2. TN-C, TNF-α, and IL-6 mRNA levels were detected by Real Time RT-PCR. Data represents mean ± SEM, as compared with control; , , and ns, nonsignificant, as compared with the cells exposed to LPS alone.

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