IL-1 Receptor Blockade Alleviates Graft-versus-Host Disease through Downregulation of an Interleukin-1-Dependent Glycolytic Pathway in Th17 Cells

Figure 5

Analysis of B cells and CD4⁺T cells in mice with acute graft-versus-host disease (GVHD), in whom splenocytes were treated with IL-1 receptor antagonist (IL-1Ra): (a) to elucidate the in vivo mechanism of action of the blockade of IL-1 signaling in amurine model of acute GVHD, we analyzed the proportion of Th1, Th2, and Th17 and (b) shows cells among ex vivo splenocytes from each group by means of flow cytometry. (c) The share of the B-cell subset was analyzed using flow cytometry. B220⁺ B cells included (immature B cells) and (mature B cells). (d) shows The level of IgG in serum. , . Data are representative of 2 independent experiments.

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