Tissue Transglutaminase-Regulated Transformed Growth Factor-β1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis

Figure 5

TGF-β1 is transcribed in Sj and Sj-infected mouse liver. Equal amounts of total RNA from left lobes of BALB/c mice liver with infective cercariae of Sj for 5, 6, 8, and 12 weeks were detected for TGF-β1 mRNA expression in Sj by using PCR (a) and SYBR Green-based quantitative PCR (b). Sj tubulin-α was detected as an input control. , . (c) Equal amounts of total RNA from infected mice livers at indicated time points were detected for mouse TGF-β1 mRNA expression by SYBR Green-based quantitative PCR. GAPDH was detected as an input control. , .

(a)

(b)

(c)