In Vivo Evaluation of the Ameliorating Effects of Small-Volume Resuscitation with Four Different Fluids on Endotoxemia-Induced Kidney Injury

Figure 1

Small-volume resuscitation alleviated the severity of renal injury in rats with LPS-induced endotoxemia. (a) and (b) show alterations in renal function parameters in response to small-volume resuscitation with different solutions after LPS administration. (c) shows Paller scores in different groups. (d) Renal pathology changes under light (stained with HE, ×400) and electron microscopy (for tubular ultrastructure, ×2950). Photomicrograph shows an intact epithelial system with normal renal tubules and glomeruli in group C, while the renal tubule exhibited serious degeneration, disintegration, and edema in epithelial cells as well as cellular debris obstructing the collecting tubules in group S (as shown by ); HTS-treated group showed only local epithelial and mitochondrial edema with less necrosis and abscission, while the HES- and HSH-treated groups only showed occasional injury. Ultrastructure (L: lumen, EC: epithelial cells). (e) Ultrastructure of glomerulus under an electron microscope (×5200). Photomicrograph shows normal glomerular structure in group C, while the number of foot processes in podocytes decreased and these foot processes were locally confluent with small amounts of dense deposits inside the basement membrane in group S. However, the podocytes were restored to a normal state with no dense deposits in photomicrographs from the HTS-, HES-, and HSH-treated groups. (L: lumen; MC: mesangial cell; RC: red cell; WC: white cell). compared to group C; compared to group S; compared to the HTS group.

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