Trimethyltin-Induced Microglial Activation via NADPH Oxidase and MAPKs Pathway in BV-2 Microglial Cells

Figure 4

TMT-induced NF-κB activation was reversed by p38 and JNK inhibitor in BV-2 cells. SB203580, SP600125, and BAY11-7082 were pretreated for 1 hr and incubated with TMT for 12 hr. (a) Protein expression of phospho-IκBα was shown by western blot. The bar graph represents the band intensity of phosphoform normalized to actin. The data are represented as mean ± SEM (). compared with the control group. (b) Following the fixation of treated cells, immunocytochemistry method was carried to observe the translocation of NF-κB p65 subunit morphologically. NF-κB p65 subunit was detected by red fluorescence (Alexa 568), nuclei were stained with blue fluorescence (Hoechst 33258), and the two different types of images were merged. The experiments were repeated more than 3 times. Scale bar: 20 μm.

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