The Expression of TLR-9, CD86, and CD95 Phenotypes in Circulating B Cells of Patients with Chronic Viral Hepatitis B or C before and after Antiviral Therapy

Figure 1

FACS analyses of B cells. Peripheral mononuclear cells were stained with anti-CD19, anti-CD38/27, anti-CD86/95, anti-IgD, anti-TLR-9, or isotype-matched IgG. The cells were gated initially on living lymphocytes (top left) and then on CD19+ B cells (lower left). Subsequently, the frequency of the activation marker (CD86) on total (CD19+), plasma (CD19+CD38+), and nonplasma (CD19+CD38−) B cells, the expression of the exhaustion marker (CD95) on total (CD19+), memory (CD19+CD27+), and naive (CD19+CD27−) B cells, and IgD as well as TLR-9 expressed on total (CD19+) B cells were analyzed by flow cytometry. At least 30,000 events were analyzed for each sample. Data are representatives of different groups of samples from at least two independent experiments.