The Expression of TLR-9, CD86, and CD95 Phenotypes in Circulating B Cells of Patients with Chronic Viral Hepatitis B or C before and after Antiviral Therapy

Figure 2

Patients with chronic HBV and HCV infections, and percentages of activated B cells and low percentages of exhausted B cells. B-lymphocytes from HCV patients (HCV, ), HBV patients (HBV, ), and healthy controls (HC, ) were analyzed by flow cytometry ex vivo for the expression of the activation marker (CD86), the exhaustion marker (CD95), and IgD. Data is shown as the frequency of the activation marker (CD86) on total (CD19+), plasma (CD19+CD38+), and nonplasma (CD19+CD38−) B cells, the expression of the exhaustion marker (CD95) on total (CD19+), memory (CD19+CD27+), and naive (CD19+CD27−) B cells, and the frequency of IgD and TLR-9 on total (CD19+) B cells stimulated with CpGB ± IL-2. Data are expressed as mean % of individual samples from at least two separate experiments. Data were analyzed by the Wilcoxon rank-sum test. The horizontal lines show the median values.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)