The Expression of TLR-9, CD86, and CD95 Phenotypes in Circulating B Cells of Patients with Chronic Viral Hepatitis B or C before and after Antiviral Therapy

Figure 4

Treatment with adefovir dipivoxil modulates the frequency of CD95+ B cells in HBV patients. A total of 15 HBV patients were treated with adefovir dipivoxil for 24 weeks, and the frequency of CD95+ B cells and IgD+ B cells in peripheral blood was determined by flow cytometry analysis at baseline and after treatment at 12 and 24 weeks: (a) the expression of the exhaustion marker (CD95) on total (CD19+), memory (CD19+CD27+), and naive (CD19+CD27−) B cells stimulated with CpGB, (b) the expression of the exhaustion marker (CD95) on total (CD19+), memory (CD19+CD27+), and naive (CD19+CD27−) B cells stimulated with CpGB+IL-2, (c) the frequency of IgD on total (CD19+) B cells stimulated with CpGB, and (d) the frequency of IgD on total (CD19+) B cells stimulated with CpGB+IL-2. Data were analyzed by the Wilcoxon rank-sum test. The horizontal lines indicate the median values of different groups. Data are expressed as mean % of individual samples from at least two separate experiments.

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