Erythropoietin Exacerbates Inflammation and Increases the Mortality of Histoplasma capsulatum-Infected Mice

Figure 3

EPO pretreatment decreases the number of mononuclear cells and increases the number of cells in late apoptosis in BALF samples of Hc-infected mice. 14 days after infection, BALF from uninfected mice treated with 45 U of EPO (EPO) and from untreated (Hc) or EPO pretreated (EPO + Hc) mice infected with Hc (5 × 10⁵ yeasts/100 μL/mouse) were evaluated for numbers of (a) mononuclear cells and (b) neutrophils. (c) Flow cytometry using annexin/PI staining was used to detect late apoptotic cells (annexin⁺/PI⁺). ((a) and (b)) Values represent means ± SEM and (c) means ± SD of one representative of two independent experiments (–6). Newman-Keuls multiple comparison test was used and the differences were considered significant when . ^#Versus Hc; uninfected and EPO-untreated (dashed line); ^&versus uninfected and EPO-treated mice.

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