Erythropoietin Exacerbates Inflammation and Increases the Mortality of Histoplasma capsulatum-Infected Mice

Figure 7

EPO pretreatment increases the area of lung tissue damage in Hc-infected mice. Lungs removed from uninfected and untreated mice (CTL) (a), EPO pretreated and uninfected mice (EPO) (b), Hc-infected only (Hc) (c), and EPO pretreated mice infected (5 × 10⁵ yeasts/100 μL/mouse) (EPO + Hc) (d). Lungs were removed 14 days after infection, processed, and stained using H&E for routine histological examination. (e) Lung-lesion area was calculated by measuring three lungs per group and was expressed as total lung area using Adobe Photoshop. Mean ± SEM values from one independent experiment are shown. Representative photomicrography of lung of one animal per group from –6. Newman-Keuls multiple comparison test was used and the differences were considered significant when . ^#Versus Hc; CTL (dashed line); ^&versus EPO.

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