Research Article
Lipoxin Inhibits Fungal Uptake by Macrophages and Reduces the Severity of Acute Pulmonary Infection Caused by Paracoccidioides brasiliensis
Figure 7
MTL treatment reduces the phagocytic ability of macrophages. For phagocytic assays, IFN-γ (20 ng/mL) unprimed (a, d) and primed (b, c, d, and f) macrophages from B10.A and A/J mice were pretreated or not with three concentrations (10−8, 10−6, and 10−4 M) of MTL. The macrophages were then infected FITC-labeled P. brasiliensis at macrophage-yeast ratio of 1 : 1 for 2 h at 37°C to allow fungi adhesion and ingestion. Macrophages were washed; cells detached from plastic and were labeled with anti-CD11b APC antibodies. The cell suspensions were immediately read on a FACSCalibur cytometer (a, b, d, and e). A quenching assay employing a trypan blue solution (TB: 250 μg/mL) was used to distinguish internalized from surface-bound yeast (c, f). Data are means ± SEM of results from quintuplicate samples from three independent experiments. .
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