Mechanisms of Thymoquinone Hepatorenal Protection in Methotrexate-Induced Toxicity in Rats

Figure 3

Effect of thymoquinone (TQ) on nuclear factor-κB (NF-κB) immunohistochemical staining of methotrexate- (MTX-) treated rat kidney and liver. Localization of NF-κB immunoreactivity (×100) in the kidney (left panel) and liver (right panel) of (a and f) control group, (b and g) TQ-treated group, (c and h) MTX-treated group, and (d and i) concomitant MTX/TQ-treated group, respectively. MTX-treated group shows immunopositivity in the nuclei of renal tubular cells (c) and hepatocytes (h) which was markedly reduced in concomitant MTX/TQ-treated group (d and i). (e and j) show semiquantitative analysis of NF-κB immunohistochemical staining results in kidney and liver, respectively. Values are represented as means ± S.E.M. difference compared with control, difference compared with MTX group. Significant difference is reported when .