Mechanisms of Thymoquinone Hepatorenal Protection in Methotrexate-Induced Toxicity in Rats

Table 1

Effect of thymoquinone (TQ) on change () of total body weight (wt), organ/total wt ratios, and kidney and liver function tests in methotrexate- (MTX-) induced toxicity.

	Control	TQ	MTX	MTX/TQ
body wt (%)	100 ± 5	99 ± 4	88 ± 2^a	98 ± 8^b,c
Kidney/wt ratio	4.9 ± 0.4	5.03 ± 0.61	6.98 ± 1.19^a	5.82 ± 0.96^b,c
Liver/wt ratio	176.5 ± 16.3	185.3 ± 33.1	369.1 ± 34.8^a	226.6 ± 26.4^b
BUN (mg/dL)	4.4 ± 0.9	3.9 ± 1.1	8.1 ± 1.8^a	5.9 ± 0.9^b,c
Creatinine (mg/dL)	0.71 ± 0.38	0.81 ± 0.27	2.19 ± 0.48^a	1.27 ± 0.72^b,c
ALT (U/dL)	24.8 ± 6.3	29.8 ± 6.2	51.9 ± 4.1^a	41.8 ± 6.3^b
AST (U/dL)	129.3 ± 24.9	149.6 ± 12.2	199.3 ± 29.1^a	162.9 ± 11.8^b

Kidney/wt and liver/wt are ratios of weight of respective organ/total body weight 1000 ratio. BUN; blood urea nitrogen, ALT; alanine aminotransferase, and AST; aspartate aminotransferase. Values are representation of 8–11 observations as means ± SEM. Results are considered significantly different when . ^aSignificant difference compared to control, ^bsignificant difference compared to MTX group, and ^cno significant difference compared to control.