Bleomycin-Treated Chimeric Thy1-Deficient Mice with Thy1-Deficient Myofibroblasts and Thy-Positive Lymphocytes Resolve Inflammation without Affecting the Fibrotic Response

Figure 1

Thy1-positive total lung cells are increased during experimental fibrosis; however, the proportion of myofibroblasts that are Thy1-positive is decreased. (A) Immunohistochemistry of Thy1 staining (arrows), in the lungs of saline- vs. bleomycin-treated mice, 14 days following instillation. Representative results. (B) Lung myofibroblasts were isolated from control saline-treated (Ctrl) and bleomycin-treated mice at 1, 3, 7 and 14 days following IT. A graphical presentation of Thy1 expression FACS analysis was performed using PE-conjugated anti-Thy1 (CD90) mAb. The differences between control (Ctrl) saline-treated and bleomycin-treated mouse lung myofibroblasts on day14 were significant. , . (C) Histogram plot showing a representative result of assessment of Thy1 expression in myofibroblasts from control (Ctrl) saline-treated mice compared to Thy1 expression in myofibroblasts from bleomycin-treated mice at day 14 following IT. Results were similar in 2 different experiments ().

(a)

(b)

(c)