Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes

Figure 2

OLN internalization by human monocytes. Human adherent monocytes (10⁶ cells/2 mL Panserin 601 medium) were left untreated (a) or treated with 200 μL rhodamine B-labeled dextran-shelled OLNs (b) for 24 h in normoxia (20% O₂). After DAPI staining, cells were checked by confocal microscopy. Results are shown as representative images from three independent experiments. Left panels: cell nuclei after DAPI staining (blue). Central panels: rhodamine B-labeled dextran-shelled OLNs (red). Right panels: merged images. Magnification: 63x.

(a)

(b)