Altered Viral Replication and Cell Responses by Inserting MicroRNA Recognition Element into PB1 in Pandemic Influenza A Virus (H1N1) 2009

Figure 1

Engineering viruses with restricted cell tropism. (a) Representative quantitative RT-PCR analysis of let-7b miRNA expression levels observed in cell lines (MDCK, HEK293, HEK293T, and HBE). Error bars denote means ± SD from triplicate experiments; similar results were obtained in three independent experiments. . (b) Schematic of cDNA base substitutions generated to transform miRNA recognition element in scbl-H1N1 or miRT-H1N1. MREs that are complementary to let-7b are in italic type, and mismatched parts are in bold. denotes amino acid substitutions.

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