Epidermal Growth Factor and Intestinal Barrier Function

Table 2

The effects of EGF on EGF-mediated protection of tight junctions.

Cell lines	Inducer	TJ associated proteins	Involved pathways	Reference
Caco-2	Hydrogen peroxide	Increased in tubulin polymerization	PKC-β1	[50]
Caco-2	Hydrogen peroxide	Increased in tubulin polymerization and decreased in monomeric tubulin	PKC-	[51]
Caco-2	Hydrogen peroxide	Increased F-actin-to-G-actin ratio	PKC-β1 ↑; NF-κB ↓^2	[52]
Caco-2	Acetaldehyde	Occludin ↑; ZO-1 ↑^1	Inhibited tyrosine phosphorylation	[53]
Caco-2	Hydrogen peroxide	F-actin ↑; G-actin ↓	PLC-γ ↑; NF-κB ↓	[40]
Caco-2	Hydrogen peroxide	Occludin ↑; ZO-1 ↑	ERK-MAPK	[23]
Caco-2	Acetaldehyde	Occludin ↑; ZO-1 ↑	Not mentioned	[54]
Caco-2	Acetaldehyde	Occludin ↑; ZO-1 ↑	PLC-γ/PKC	[55]
Caco-2	Acetaldehyde	Occludin ↑; ZO-1 ↑	ERK1/2-MAPK	[24]
NRC-1 cells^3	Hydrogen peroxide	ZO-1 ↑; claudin-3 ↑	PLC-γ/PKC	[56]
MCAS^4	None	Claudin-3 ↓	MEK/ERK or; PI3K/Akt	[57]
HUOA^5		Claudin-4 ↓
MDCK^6	None	Claudin-4 ↑	MEK/ERK	[58]
MDCK	None	Claudin-2 ↓; claudin-4 ↑	Src and STAT3	[25]

Symbols ↑ and ↓ indicate increases and decreases in the protein or mRNA expression, respectively. ²Symbols ↑ and ↓ stand for activation and inhibition, respectively. ³Cholangiocytes. ⁴Mucinous cystadenocarcinoma. ⁵Serous cystadenocarcinoma. ⁶Darby canine kidney cells.