Inhibition of Macrophage Migration Inhibitory Factor Protects against Inflammation and Matrix Deposition in Kidney Tissues after Injury

Figure 2

Recanalization reduced matrix deposition and MIF expression in obstructed kidneys. (a) PAS and Masson staining revealed that recanalization operation reduced obstruction-induced kidney injury, matrix deposition, and inflammatory cells infiltration in BUO rats. Bar: 50 μm. (b) Immunohistochemical staining showed that recanalization downregulated obstruction-induced protein expression of type III collagen. Bar: 100 μm. (c) Gene expression levels of Col1α1 and Col3α1 in BUO kidneys were significantly decreased after recanalization. (d) ELISA assay indicated reduced TGF-β1 levels in recanalization kidneys compared with the BUO group. (e) Western blot analysis showed decreased MIF expression in recanalization kidneys. (f) qRT-PCR analysis showed reduced MIF expression in recanalization kidneys compared with the BUO group. (g) Immunohistochemical staining identified downregulated expression of MIF and CD74 in kidneys of recanalization rats. Bar: 50 μm. (h) Recanalization inhibited the infiltration of CD68-positive macrophages in BUO kidneys. Bar: 50 μm.

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