Shikonin Attenuates Concanavalin A-Induced Acute Liver Injury in Mice via Inhibition of the JNK Pathway

Figure 5

Effects of shikonin on the regulation of the IL-1β/JNK/p-JNK pathway in mice with ConA-induced acute hepatitis. (a) The levels of proteins IL-1β, total JNK, and p-JNK in liver tissue are shown as western blot bands and the gray values were calculated (, for NC versus ConA, for ConA + shikonin (7.5) versus ConA, and for ConA + shikonin (12.5) versus ConA). (b) The expression of p-JNK in hepatic tissues was determined with immunohistochemistry at 6 h (original magnification, ×200). The ratio of brown area to total area was analyzed with Image-Pro Plus (v 6.0) (, for NC versus ConA and for ConA versus ConA + shikonin). (c) The generation of ROS was detected with ROS Fluorescent Probe-DHE. And ROS were measured and analyzed in three random vision fields by Image-Pro Plus 6.0 (, for NC versus ConA and for ConA + shikonin versus ConA).

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