M1 and M2 Functional Imprinting of Primary Microglia: Role of P2X7 Activation and miR-125b

Figure 1

Microglia responsiveness to macrophages polarity gradient. Primary microglia obtained from brain cortex of P0/P1 C57BL/6 mice were prepared as previously described [15]. (a) TNFαor (b) MRC-1 expression levels were measured by qRT-PCR using primer pairs from Qiagen-GeneGlobe program. Data are calculated using the method and GAPDH transcript as normalizer (Qiagen-GeneGlobe program). Bars represent mean ± sem of ≥3 independent experiments; , , and versus untreated cells by unpaired -test. (c) Schematic representation of the response of primary microglia to polarity gradient protocol used as revised from [34].

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