Depletion of Neutrophils Exacerbates the Early Inflammatory Immune Response in Lungs of Mice Infected with Paracoccidioides brasiliensis

Figure 2

Depletion of neutrophils is associated with an exacerbated inflammatory response in lungs of mice infected with P. brasiliensis during the acute phase. The microphotographs shown are representative of lungs from mice infected or noninfected and treated or not with the anti-Ly6G specific mAb against neutrophils at 96 h after challenge and obtained from 4-5 mice/group. Lungs were fixed, embedded in paraffin, cut, and stained using H&E staining (a–g) to determine lung inflammatory response and methenamine silver staining (h–n) to identify P. brasiliensis yeast cells as described in the Materials and Methods section. Arrowheads indicate P. brasiliensis yeast cells (in brown). These results are representative of two independent experiments. (a) and (h) Control mice (inoculated with PBS); (b), (e), (i), and (l) infected, untreated mice; (c), (f), (j), and (m) infected mice treated with isotype control Ab; (d), (g), (k), and (n) infected mice treated with anti-Ly6G mAb. Magnification 40x (a–d, h–k) and 400x (e–g, l–o).