Epstein-Barr Virus-Induced Gene 3 (EBI3) Blocking Leads to Induce Antitumor Cytotoxic T Lymphocyte Response and Suppress Tumor Growth in Colorectal Cancer by Bidirectional Reciprocal-Regulation STAT3 Signaling Pathway

Figure 2

The natural EBI3 signal blocking effect of EBI3 blocking peptide. (a) Mouse splenic lymphocytes (SLs) were isolated from Balb/c mice, SLs were cocultured with or without the indicated doses of EBI3 blocking peptide (EBI3 Bp) in the presence of ConA (1 μg/mL), and SLs proliferation were detected by CFDA assay. (b) Bone marrow from femurs and tibias of Balb/c mice was cultured in RPMI1640 supplemented with 10% FBS and 10 ng/ml murine M-CSF for 6 days and used as mouse bone marrow-derived macrophages (BMDMs). BMDMs pretreated with or without EBI3 Bp for 10 min and then stimulated with LPS for additional 1 day. Culture supernatants were used for SLs culture and after 3-day culture, IFN-γ producing SLs were analyzed by FCM. ; .

(a)

(b)