Epstein-Barr Virus-Induced Gene 3 (EBI3) Blocking Leads to Induce Antitumor Cytotoxic T Lymphocyte Response and Suppress Tumor Growth in Colorectal Cancer by Bidirectional Reciprocal-Regulation STAT3 Signaling Pathway

Figure 4

Assessing the role of EBI3 blocking in CRC cell proliferation and tumor growth in vitro and in vivo. (a) CRC cell proliferation after stimulation with EBI3 Bp or coculture with autologous TILs was detected by CCK-8, an isotype IgG, and PBS for control. (b) Supernatants IL-35 levels were detected by ELISA. (c) Supernatants IL-27 levels were detected by ELISA. (d) Representative images of the tumors and spleens at the endpoint from tumor xenograft models. (e) Tumor volumes were measured at the endpoint. (f) Spleens weight was measured at the endpoint. Significant decrease in tumor size but not spleens weight was observed in mice treated with EBI3 Bp as compared to those of control mice. ; .

(a)

(b)

(c)

(d)

(e)

(f)