Epstein-Barr Virus-Induced Gene 3 (EBI3) Blocking Leads to Induce Antitumor Cytotoxic T Lymphocyte Response and Suppress Tumor Growth in Colorectal Cancer by Bidirectional Reciprocal-Regulation STAT3 Signaling Pathway

Figure 5

EBI3 regulates the tumor growth and antitumor cytotoxic T lymphocyte response by bidirectional reciprocal-regulation STAT3 signaling pathway. (a) Representative images for the gp130 and p-STAT3 expression on CD45⁺CD3⁺CD8⁺ TILs after being cocultured with CRC cells stimulated with EBI3 Bp for 6 h, an isotype IgG, and PBS for control. Scatter plot graph showing the percentage of gp130⁺p-STAT3⁺ T cells on CD45⁺CD3⁺CD8⁺ TILs. (b) The expression level of gp130 and p-STAT3 was detected by Western blot analysis. (c) gp130 and p-STAT3 in tumor of xenograft models were detected by immunohistochemistry. Low expression of gp130 and p-STAT3 was observed in mice treated with EBI3 Bp as compared to those of control mice. .

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