A Comparative Study of the T Cell Stimulatory and Polarizing Capacity of Human Primary Blood Dendritic Cell Subsets

Figure 1

Phenotype of human blood DCs upon TLR stimulation. (a) MHC class I (HLA-ABC) and MHC class II (HLA-DR) expression was analyzed by flow cytometry of DCs kept at 4°C or DCs stimulated and cultured overnight. Myeloid DCs were stimulated with R848 and polyI:C (pI:C), whereas pDCs were stimulated with R848 alone. The bar graphs show the mean ± SEM of the mean fluorescent intensity (MFI) () and the histogram shows expression of freshly isolated DC subsets from a single representative donor (filled histogram: isotype control; grey line: pDCs, black line: CD1c⁺ mDCs, dashed line: CD141⁺ mDCs). (b) The bar graphs (upper panel) show the fold change ± SEM of the MFI for surface expression of the costimulatory molecules CD80 and CD86 after overnight stimulation with reference to cells cultured in medium alone (or IL-3 for pDCs) (). The histograms (lower panel) show CD80 and CD86 expression from a single representative donor (insets: MFI values). Significance was determined by Kruskal-Wallis test followed by Dunn’s testing comparing different conditions of the same subset (; ; ).

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