Clinical Study
Circulating Memory T Follicular Helper Cells in Patients with Neuromyelitis Optica/Neuromyelitis Optica Spectrum Disorders
Figure 4
FACS analysis of circulating CCR7+ and CCR7+ICOS+ memory Tfh cells in individual participants. After staining with different fluorescent antibodies, the cells were gated sequentially on lymphocytes, CD3+ and CD4+, and then CD45RAā and CCR7+ cells. After that, the cells were gated on CXCR5, and we obtained CD3+CD4+CD45RAāCCR7+CXCR5+ (CCR7+ memory Tfh) cells. Subsequently, CCR7+ memory Tfh cells were gated on ICOS expression, and the percentages of CCR7+ and CCR7+ICOS+ memory Tfh cells were analyzed by flow cytometry. The numbers of each subtype of cells were calculated. (a) Flow cytometric analysis. ((b)-(c)) The percentages of circulating CCR7+ and CCR7+ICOS+ memory Tfh cells in HCs and untreated NMO/NMOSD patients. ((d)-(e)) The numbers of circulating CCR7+ and CCR7+ICOS+ memory Tfh cells in HCs and untreated NMO/NMOSD patients. The horizontal lines indicate the median values for each group.
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