Adrenal-Derived Hormones Differentially Modulate Intestinal Immunity in Experimental Colitis

Figure 3

Adrenal-derived mediators modulate local tissue damage. C57BL/6 mice were adrenalectomized and exposed to dextran sulfate sodium (DSS) 3%. The colon was collected on the 6th day of colitis for histological analysis (a) in (b) immunophenotyping of CD11b⁺ cells, (c) dendritic proinflammatory cells (CD11b⁺CD11c⁺CD103⁻), and (d) tolerogenic dendritic cells (CD11b⁺CD11c⁺CD103⁺), evaluated by flow cytometry. (e) Myeloperoxidase (MPO) and (f) eosinophil peroxidase (EPO) assays. DSS: C57BL/6 mice exposed to DSS; ADX + DSS: C57BL/6 adrenalectomized mice exposed to DSS. The dotted lines correspond to the control group of C57BL/6 mice without colitis. Results are representative of two independent experiments, with 5 mice/group. .

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