Elevated Plasma Level of Interferon-λ1 in Chronic Spontaneous Urticaria: Upregulated Expression in CD8⁺ and Epithelial Cells and Induction of Inflammatory Cell Accumulation

Figure 6

Interferon- (IFN-) λ1 induces inflammatory cell accumulation in mouse peritoneum. Mice were treated with IFN-λ1 (ng/mL) in the presence or absence of its specific antibody (3.0 μg/mL), eotaxin (10 ng/mL), human serum albumin (HSA, 10 ng/mL), lipopolysaccharide (LPS, 1.0 μg/mL), and normal saline (NS) for 6 h before their peritoneal lavage being collected for differential cell analysis. For certain mice, anti-CD54 antibody (anti-CD54, 1 mg⋅kg⁻¹) and anti-CD62L antibody (anti-CD62L, 1 mg⋅kg⁻¹) were injected via tail vein 30 min before IFN-λ1 being injected. Data were displayed as a boxplot, which indicates the median, interquartile range, the largest, and smallest values other than outliers (whiskers) and the outliers (O). Each piece of data represented a group of 6-7 animals. compared with the corresponding NS group. compared with the corresponding stimulus alone group.

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