An EP2 Agonist Facilitates NMDA-Induced Outward Currents and Inhibits Dendritic Beading through Activation of BK Channels in Mouse Cortical Neurons

Figure 1

Potentiation of NMDA-induced outward currents through the activation of EP2 receptors in the acutely dissociated mouse cortical neurons. (a) Superimposed traces show NMDA-induced currents in the absence (black traces) or the presence (red traces) of drugs. PGE₂ (10 μM), ONO-DI-004 (an EP1 receptor agonist, 10 μM), ONO-AE1-259 (an EP2 receptor agonist, 10 μM), ONO-AE-248 (an EP3 receptor agonist, 10 μM), and ONO-AE1-329 (an EP4 receptor agonist, 10 μM) were simultaneously applied with NMDA (30 μM). A black line indicates the drug application period. Broken lines indicate basal currents. Calibration bars are 50 pA and 20 sec. (b) Dose response of EP receptor agonists on NMDA-induced outward currents. The symbols and bars represent the mean ± SEM ( = 5–7 cells in each responses). Asterisks indicate a significant difference from 0 μM (, , and , one-way ANOVA with post hoc Dunnett’s test). Swords indicate a significant difference between PGE₂ and ONO-AE1-259 (, = 47.28, two-way ANOVA Bonferroni test).

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