Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 and MMP-9 Expression in the Mouse Colon

Table 1

Primers sequence for qPCR.

Species	mRNA	Gene	Primer sequence		Amplicon (bp)
M	NM-031168.1	IL-6	Forward	5′-AACGATGATGCACTTGCAGA	128
			Reverse	5′-TGGTACTCCAGAAGACCAGAGG
M	NM-011339.2	IL-8	Forward	5′-CTAGGCATCTTCGTCCGTCC	217
			Reverse	5′-TTGGGCCAACAGTAGCCTTC
M	NM-013693.3	TNF-α	Forward	5′-CCACCACGCTCTTCTGTCTACT	161
			Reverse	5′-TGCTACGACGTGGGCTACA
M	NM-008610	MMP-2	Forward	5′-ACAAGTGGTCCGCGTAAAGT	189
			Reverse	5′-GTAAACAAGGCTTCATGGGGG
M	NM-010809.1	MMP-3	Forward	5′-AGGGATGATGATGCTGGTATG	210
			Reverse	5′-AACACCACACCTGGGCTTAT
M	NM-013599	MMP-9	Forward	5′-GCCGACTTTTGTGGTCTTCC	80
			Reverse	5′-GGTACAAGTATGCCTCTGCCA
M	NM-001044384	TIMP-1	Forward	5′-GACACACCAGAGCAGATACCAT	189
			Reverse	5′-TGGTCTCGTTGATTTCTGGGG
M	NM-011594	TIMP-2	Forward	5′-GTGCAAGATCACTCGCTGTC	102
			Reverse	5′-TGGTGCCCATTGATGCTCTT
M	NM-007393	β-Actin	Forward	5′-GGCTGTATTCCCCTCCATCG	154
			Reverse	5′-CCAGTTGGTAACAATGCCATGT
H	NM-002422.3	MMP-3	Forward	5′-AGGGATGATGATGCTGGTATG
			Reverse	5′-AACACCACACCTGGGCTTAT	174
H	NM-004994.2	MMP-9	Forward	5′-TTCAGGGAGACGCCCATTTC
			Reverse	5′-TGGGTGTAGAGTCTCTCGCT	244
H	NM-001101	β-Actin	Forward	5′-CTCTTCCAGCCTTCCTTCCT
			Reverse	5′-AGCACTGTGTTGGCGTACAG	116

M: mouse, H: human.