Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-α Production in PBMC of Patients with Behçet’s Disease

Figure 1

Expression of SFKs in PBMC of active BD patients. PBMC were isolated from total blood and 5 × 10⁶ PBMC were cultured. The cells were treated with 10 μM PP2 for 1 h before 100 ng/mL LPS stimulation for 15 min. DMSO treated cells were used as control. Cell lysates were examined for the presence of SFKs and activity of SFKs by Western Blotting. The lowest panel represents loading control (β-actin). The image shown represents a single representative example of five separate experiments.