Exaggerated IL-15 and Altered Expression of foxp3+ Cell-Derived Cytokines Contribute to Enhanced Colitis in Nlrp3−/− Mice

Figure 4

BM-derived DCs from Nlrp3−/− mice exhibit increased frequency of IL-15 expression an effect that cannot be normalized by exogenous IL-1. (a) Flow cytometric analysis of IL-15 expression on the surface of WT and Nlrp3−/− DCs differentiated from BM progenitor cells for 10 days with Flt3L (100 ng/mL), expressed as a percentage of IL-15 expressing cells (a) and MFI per cell (b). (c) Flow cytometric analysis of IL-15 surface expression on WT and Nlrp3−/− DCs differentiated from BM progenitor cells for 10 days with Flt3L (100 ng/mL) and treated with IL-1 (100 ng/mL) and/or recombinant IL-1 receptor antagonist (IL-1Ra; 100 μg/mL) for 12 hours. denotes compared to WT mice; # denotes compared to WT, NoRx; denotes compared to WT-IL-1; /group.

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