Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKK-AMPK-SIRT1 Signaling Pathway

Figure 1

LPS induces the low levels of IL-12 in murine peritoneal macrophages. (a) Murine peritoneal macrophages were treated with or without 100 ng/mL LPS for 24 h. Supernatant levels of TNF-α, IL-6, IL-10, IL-12 p40, and IL-12 p70 were detected by ELISA. (b, c) Peritoneal macrophages were treated with LPS (0, 10, 100, and 1000 ng/mL) for 24 h (b) or with 100 ng/mL LPS for 3, 6, 12, and 24 h (c). Supernatant levels of TNF-α and IL-12 p40/p70 were detected by ELISA ().

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(b)

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