Blockade of Wnt/β-Catenin Pathway Aggravated Silica-Induced Lung Inflammation through Tregs Regulation on Th Immune Responses

Figure 3

Blockade of Wnt/β-catenin pathway increased the production of proinflammatory cytokines. (a) Expression of IL-1β mRNA in lung was assayed by real-time RT-PCR using the method. The concentrations of TNF-α (b) and IL-6 (c) in BALF were assayed by flow cytometry using CBA Kit. Values are shown as mean ± SEM (-5) (, as compared with saline group at the same time point, ; #, as compared with silica group and silica + NC shRNA group at the same time point, ).

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