Blockade of Wnt/β-Catenin Pathway Aggravated Silica-Induced Lung Inflammation through Tregs Regulation on Th Immune Responses

Figure 5

Th1 response was enhanced by blocking the Wnt/β-catenin pathway. Expressions of IFN-γ (a) and T-bet (b) in lung were assayed by real-time RT-PCR using the method. (c) The percentage of IFN-γ⁺CD4⁺ T cells in HLN was calculated by flow cytometry; data were shown as scatter plot and graph. Values are shown as mean ± SEM (-5) (, as compared with saline group at the same time point, ; #, as compared with silica group and silica + NC shRNA group at the same time point, ).

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