15-Lipoxygenase-1 Is Involved in the Effects of Atorvastatin on Endothelial Dysfunction

Figure 5

The relationship between atorvastatin and ALOX15. (a) ox-LDL (100 mg/L) increased NF-κB activity in a time-dependent manner. (b) and (c) NF-κB activity affected ox-LDL-induced expression of ALOX15 in HUVECs, which were preincubated with pyrrolidine dithiocarbamate (PDTC) (1 μM to 100 μM) for 1 h before ox-LDL stimulation. (d) HUVECs were preincubated with atorvastatin (10 μM) for 8 h before ox-LDL stimulation, after which NF-κB-p65 protein levels were examined by western blot analysis. (e) and (f) Effects of atorvastatin and PDTC on ALOX15 protein expression and enzyme activity in HUVECs. Results are expressed as mean ± SD. NC: normal control; NS: not significant. or 4 per group.

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