2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis

Figure 4

THSG attenuated P. gingivalis LPS-induced proinflammatory cytokines expression via reducing NF-κB activation and activating AMPK and ERK1/2 in HGFs. HGFs were cultured in 10-cm dishes and starved for 24 h when the cell confluence reached 80%. HGFs were pretreated with 100 μM resveratrol and THSG for 30 min and then treated with 1 μg/mL P. gingivalis LPS for another 3 h. (a) The cytosolic and nuclear proteins of HGFs were extracted and Western blot was performed. (b) The relative intensity of nuclear translocation of NF-κB and activation of AMPK and ERK1/2 were evaluated. (; ; ; were compared with control group. ; were compared with LPS group.)

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