Circulating (CD3⁻CD19⁺CD20⁻IgD⁻CD27^highCD38^high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy?

Figure 2

Time-course of renal function parameters (a) plasma creatinine and 24 h-proteinuria levels under immunosuppressive therapy and (b) values of serum anti-PLA2R1 antibody levels. (c and d) Representative pictures of immunofluorescence of phospholipase A2 receptor expression within the glomerulus performed on formalin-fixed and paraffin embedded kidney tissue section obtained from a first (c) and a second (d) kidney biopsy in patient with membranous nephropathy. Confocal microscopic analysis of a paraffin kidney biopsy specimen revealed the presence of PLA2R1 in subepithelial deposits along glomerular capillary loops (white arrows), ×400 magnification. Kindly performed by Hanna Debiec INSERM U702, Hôpital Tenon, Paris, France.