Circulating (CD3⁻CD19⁺CD20⁻IgD⁻CD27^highCD38^high) Plasmablasts: A Promising Cellular Biomarker for Immune Activity for Anti-PLA2R1 Related Membranous Nephropathy?

Figure 4

Representative pictures of intrarenal inflammatory cells identified by immunoperoxidase and double immunofluorescence stainings in tissue samples provided from second kidney biopsy. Kidney tissue sections were probed with antibodies against the following antigens: CD8 (cytotoxic T cells), CD4 (helper/suppressor T cells), CD68 (monocytes/macrophages), and CD20 (B cells). One of the two small B cell follicles, which corresponded to tertiary lymphoid organs contained CD21+ follicular dendritic cells indicating the presence of germinal center activities despite the absence of the B cell attracting chemokine, CXCL13 within the germinal center. Original magnifications: (a–d) ×200 and (e-f) ×1000.