Effects of Chinese Propolis in Protecting Bovine Mammary Epithelial Cells against Mastitis Pathogens-Induced Cell Damage

Figure 4

Time and dose effects of CP treatment on inflammatory cytokine and cellular antioxidant defense gene expressions in TNF-α stimulated MAC-T cells. MAC-T cells were preincubated with different concentrations of propolis for 1 h and then stimulated with 25 ng/mL TNF-α for various time periods (time effects, right) or preincubated with various concentrations of propolis (dose effect, left) and then stimulated with 25 ng/mL TNF-α for 6 h. IL-6 (a), IL-8 (b), TNF-α (c), HO-1 (d), Txnrd-1 (e), and GCLM (f) mRNA expressions were quantified by real-time PCR. Ct values of target genes were normalized to the value of β-actin. Relative gene expression in TNF-α stimulation for 6 h was arbitrarily set to one. The data are shown as mean ± SD from three independent experiments. ^# compared to the normal control and different from the TNF-α treated control (). , , and .

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